Systems NMR integrates Nuclear Magnetic Resonance spectroscopy and mathematical modeling for quantitative visualization and analysis of medium-sized biomolecular networks (dozens of reactions).

Systems NMR vs Omics and Biochemistry

Key features of the method:

  1. Combines dynamic resolution of biochemical assays and multiplexing ability of “omics” methods. This permits quantification of multiple network fluxes using single-sample measurements. Other ways of obtaining the same information require either multiple biochemical experiments - each resolving dozens of time-points for one network component in one sample, or multiple “omics” experiments - each resolving concentrations of multiple molecules, but only at one or a few time-points at a time.

  2. Permits to quantify in one sample at the same time: all elementary reaction types (catalysis, bimolecular interactions, unimolecular state changes) and soluble biomolecule classes - e.g. Metabolites, Proteins, RNAs (provided these are ≥ 10-50 µM in concentration and ≤ 50-100 kDa in size).